FIT screening

Dikkedarmkanker, ook wel colorectaal carcinoom, is een veelvoorkomende ziekte onder voornamelijk ouderen. Het detecteren van dikke darmkanker in een vroeg stadium kan de overlevingskans van een patiënt aanzienlijk vergroten. Door gebruik te maken van een fecale immunochemische test (FIT) worden kleine hoeveelheden bloed (met behulp van antilichamen voor humaan hemoglobine) opgespoord in ontlastingsmonsters.

Sysmex biedt FIT-tests aan voor twee hoofdgroepen: grootschalig tests voor nationale of regionale bevolkingsonderzoeken, en symptomatische tests die hoofdzakelijk plaatsvinden in ziekenhuizen of klinische omgevingen. Lees hier meer over op onze FIT screening microsite

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Questions regarding the OSNA® method

What does the abbreviation OSNA mean?

OSNA® stands for »one-step nucleic acid amplification.« The method involves the homogenisation of lymph node tissue followed by analysis of a molecular target mRNA called CK19 by the process of »reverse-transcription-loop-mediated-isothermal-amplification« (RT-LAMP). The name OSNA® is proprietary by Sysmex.

How does the LAMP-reaction work in principle?

LAMP which stands for Loop-mediated Isothermal Amplification is a simple, rapid and specific nucleic acid amplification method solely developed by Eiken Chemical Co., Ltd. It is characterised by the use of 6 different primers specifically designed to recognize 6 or 8 distinct regions on the target sequence. The reaction utilises a DNA Polymerase with strand displacement activity and proceeds at a constant temperature of around 65°C. Amplification and detection of the target sequence can be completed in a single step.

What are the characteristics of OSNA®?

The method is characterised by

  • No requirement for an RNA purification step.
  • A short reaction time consistent with the intra-operative use of the system.
  • The use of 6 primers that lead to a high specificity for the target mRNA.
  • Optimal Assay Design avoiding the amplification of CK19 Pseudogenes.
  • Gene-amplification at a constant temperature of 65°C to avoid genomic DNA amplification.

How long does an amplification reaction take on the RD100i Robotic Analyser System?

It takes 16 minutes.

How fast is the whole procedure of a sample analysis?

It takes about 30 minutes including pre-treatment for one lymph node and 40 minutes for 4 lymph nodes.

How many lymph nodes can be analyzed simultaneously?

Up to 4 lymph nodes can be processed in one run.

Can remaining lymph node lysate be stored?

The lysate can either be stored at -80°C or in liquid nitrogen.

Does the blue dye interfere with the OSNA® reaction?

The blue dye used in the surgical identification of Sentinel Lymph Nodes does not have an impact on the OSNA® result.

Questions regarding the reagents

What is the storage temperature of the reagents (Lynorhag and Lynoamp)?

Please store the homogenising reagent (Lynorhag) at 4°C and the amplification kit (Lynoamp) at -20°C.

How often can I thaw and freeze primers, controls and calibrator solutions?

The solutions may be freeze thawed upto 5 times.

How long can the reagents be kept outside the freezer – either on ice or in the instrument?

LynoAmp reagents can be kept on ice or on board the RD100i Robotic System for about 6 hrs.

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