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Recycling Preparative HPLC

For preparative HPLC, the column length is one of the keys to improve separation. However, there is a limit as to the length due to back pressure problem. To solve this, recycling technique can be applied. After initially passing through the columns, the sample is recycled by returning to the same columns and the operation is repeated until baseline separation is achieved. With recycling technique, the chromatographic resolution increases by the square root of number of passes through the column and there is no solvent consumption. Recycling conserves solvent use and reduces the investment in columns. This is the method you've been looking for!  

Field of usage of preparative recycling HPLC:       

 

  • Separation and Fraction at organic synthesis research institute.  JAI recycling preparative HPLC is widely chosen at many organic synthesis fields to separate target compound from impurities and purify the target substance as the most effective method, while saving times and finding new approach.  
  • Isolation of biologically active substance.  Recycling technique is also a very effective method for purification and isolation of biologically active substance and to collect large volume of active ingredient at pharmaceutical development.    

 

If you are unsure whether the recycling prep HPLC system works for your application, we can arrange for a trial run with your samples. Contact us by email or phone for details.

Chromatography

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