Sysmex Nederland


  • Most sensitive light scattering detector in the world
  • Unrivalled beseline stability on all detectors for greater sensitivity to low concentrations of sample
  • Self balancing viscometer detector
  • Wide UV/VIS wavelength range to cover all applications
  • Simple and intuitive to use, due to advanced design features


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The ultimate in integrated advanced detection for GPC/SEC and UPLC/UHPLC

Molecular weight, intrinsic viscosity and numerous other parameters can be measured with OMNISEC REVEAL, the world’s most advanced integrated GPC/SEC multi-detector available. It can be added to any existing conventional GPC/SEC system or used in conjunction with OMNISEC RESOLVE to form a complete GPC/SEC solution, to offer comprehensive molecular characterization of synthetic and natural polymers, as well as proteins.

Multiple applications
OMNISEC REVEAL can be used to relate a polymer’s molecular properties to its bulk characteristics in a wide range of applications, from the packaging and paint industries to the manufacturing of food additives and pharmaceuticals. Its protein characterization capabilities can be used to study the effects of oligomerization, aggregation and conjugation on activity and efficacy in academic research and biopharmaceutical development.

With OMNISEC REVEAL you can:

  • Understand and control product performance of polymers, polysaccharides and proteins by measuring absolute molecular weight, intrinsic viscosity, hydrodynamic radius, Mark-Houwink parameters, radius of gyration, branching, and co-polymer composition.
  • Measure molecular weight as low as 200 Da or injection masses as low as 100 ng for characterization of samples which were previously unmeasurable using advanced detection.
  • Improve separations and result accuracy with the column oven and temperature-controlled integrated detector compartment that minimizes inter-detector tubing

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Dimensions: 420 (W) x 640 (D) x 600 (H) mm
Weight: 40 kg
Detector temperature range: 20 - 65 °C

Detector 1
Detector: Light scattering
Measurement principle: RALS 90° angle, LALS 7° angle
Light source: 50 mW, 640 nm laser
Cell volume: 18 µL
Dynamic range:2500 mV 
Baseline noise: <0.1 mV
Baseline drift: <0.2 mV/hr

Detector 2
Detector: Differential refractive index
Measurement principle: Deflection
Cell volume: 12 µL 
Dynamic range: ±2.5 x 10-4 RIU 
Baseline noise:<10-7 RIU
Baseline drift:<3 x 10-7 RIU/hr

Detector 3
Detector: Viscometer
Measurement principle: 4-capillary 
Wheatstone bridge with self-balancing mechanism and user-exchangeable capillaries
Detector volume:17 µL/capillary
Differential pressure dynamic range:±2500 Pa
Differential pressure baseline noise:0.3 Pa
Inlet pressure dynamic range:100 kPa
Inlet pressure baseline noise:0.01 kPa
Baseline drift:<0.2 kPa/hr

Detector 4
Detector: Diode-array-based UV/Vis spectrometer
Wavelength: 190 - 900 nm
Number of wavelengths: 1024
Wavelength accuracy: <1 nm
Wavelength resolution:0.6 nm
Cell volume: 7.5 µL
Path length:10 mm
Baseline noise:2 x 10-5 AU
Baseline drift:5 x 10-4 AU/hr

Measurement type 1
Measurement type: Absolute molecular weight
Measurement range:200 - >107 g/mol
Minimum quantifiable mass: 100 ng of 100kDa molecular weight polystyrene in THF
Measurement principle: Light scattering (LALS or RALS)

Measurement type 2
Measurement type: Intrinsic viscosity
Minimum quantifiable mass: 1 µg of 100kDa molecular weight polystyrene in THF
Measurement principle: 4-capillary Wheatstone bridge

Measurement type 3
Measurement type: Concentration
Minimum quantifiable mass: 100 ng of 100kDa molecular weight polystyrene in THF
Measurement principle: Differential refractive index detection

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