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Malvern Zetasizer APS

  • Investigate your protein over a large number of environmental conditions with minimal user intervention
  • Uses industry standard 96 or 384 well plates
  • Measure aggregation temperature (melting point) to predict or compare stability
  • 2 - 90°C temperature range for measurement and 4 - 40°C temperature range for protein storage
  • Assess purity to screen for suitable crystallization buffer

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The highest accuracy automated protein size measurement

The Zetasizer APS is a robust, simple to operate, Dynamic Light Scattering automated plate sampler that automates the measurement of samples in industry standard 96- or 384-well plates. Dual temperature control enables the well plate to be kept at a low temperature to preserve the protein before measurement.

The Zetasizer APS transfers 20µL sample from each well to a precision quartz flow cell for measurement. The cell environment is optimized for precision temperature control and optical clarity to ensure the most accurate and precise results. This means that any industry standard disposable or reusable plate can be used without concern for transparency or scratches on the plate that would affect the result.

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Specifications

Specifications

Particle size and molecular size

Measurement range
Particle size and molecular size

Measurement principle
Dynamic Light Scattering

Minimum sample volume
20µL

Accuracy
Better than +/-2% on NIST traceable latex standards

Precision/Repeatability
Better than +/-2% on NIST traceable latex standards

Sensitivity
0.1mg/mL 15kDa protein (Lysozyme)

Aggregation temperature (melting point)

Measurement range
2ºC - 90°C +/- 0.1

Measurement principle
Dynamic light scattering

Minimum sample volume
20µL

Temperature control range
2ºC - 90ºC +/- 0.1

Compatible plate types
96, 384 well (SBS standard) †

Light source
Laser 830nm, max 60mW

Laser safety
Class 1 

Dimensions
500 (W) x 410 (D) x 420 (H) mm

Weight
22.5kg

How it works

How it works

The Zetasizer APS (Automated Plate Sampler) uses laser light scattering to determine a number of parameters that are associated with protein conformation and stability.
The system integrates a plate sampling system and measurement unit into a compact box to simplify use.

Dynamic Light Scattering (DLS) determines size by measuring the speed of Brownian movement of particles or molecules in a solvent or buffer. This diffusion speed is converted into a size, polydispersity and size distribution with knowledge of the temperature and buffer viscosity using the Stokes-Einstein relationship.

The size is used to determine oligomeric state, polydispersity provides the purity, and the size distribution is used to detect aggregates. Light scattering is very sensitive to the presence of aggregates, so is an ideal technique to determine the early stages of instability.

Accessoiries

Accessoiries

PTC-1 Plate temperature control unit
Maintains the well plate at a constant temperature (- 4°C to 40°C), primarily to preserve the protein before measurement.

SV-10 viscometer
The SV-10 measures viscosity with the accuracy required for dynamic light scattering, for the conversion of diffusion speed to size, and electrophoretic mobility to zeta potential, in an extremely simple and rapid system.

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