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CyFlow Space

  • Configurations with up to 13 fluorescence parameters and 3 physical light scattering parameters
  • Choice of lasers with 365, 375, 405, 488, 532, 561, 594, 638, 640 nm
  • Maximum acquisition rate up to 25,000 signals/s
  • Scatter particle size: 0.1 – 100 µm
  • Fluorescence sensitivity: < 100 MESF (FITC), < 50 MESF (PE)

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Flexibility that gives you the space you need for your work

The CyFlow Space is a high-performance multi-laser flow cytometer, which offers the most flexible, simple and reliable features for routine and research work. It is a multi-colour instrument for the analysis of individual cells and microscopic particles in suspension. The advanced flow cytometry technology of the CyFlow Space covers a wide range of different applications.

Parameters
Fluorescence excitation by one to five light sources covering a range from ultra-violet to red excitation lines. o Analysis of up to 13 fluorescence parameters and three physical light scattering parameters from near UV to infra-red light.

TVAC
Analysis of cell and particle concentration by True Volumetric Absolute Counting (TVAC).

Cell sorting
Cell sorting by an optional cell and particle sorter.

Automation
Walk-away automation by sample auto-loading from 96- and 384-well plates.

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Specifications

Specifications

Light sources (Up to five light sources)
Blue solid state laser: 50mW fix or 200mW adjustable at 488nm
Red diode laser: 25mW at 638nm or 40mW at 640nm
Violet diode laser: 100mW at 405nm
Green DPSS laser: 30 or 100mW at 532nm
Yellow laser: 100mW at 561nm
High-power UV LED: 365nm
Ultraviolet diode laser: 60mW at 375nm
Orange diode laser: 50mW at 594nm

Optics
Modular optical system with up to 16/eight optical parameters with selected PMTs with integrated electronic preamplifier for FSC, SSC, FL1-FL13
Exchangeable optical filters
Colour CCD camera for sample flow monitoring

Flow system
Quartz flow cuvette for laminar sample transport and hydrodynamic focussing
True Volumetric Absolute Counting (TVAC) based on mechanical volume measurement
Computer-controlled precision syringe pump, speed continuously adjustable from 0-20 µL/s

Dimensions
560 (W) x 300 (H) x 650 (D) mm

Software

Software

FloMax flow cytometry data acquisition and analysis software
The Windows™-based FloMax software includes multiple online gating, logical gates, and colour gating. N-colour online and offline software compensation is another standard feature of the FloMax software. Data may be acquired with compensation or as uncompensated raw data. Either way, compensation can be done, undone, or changed after analysis on stored data. User-selectable statistics are calculated on subset populations identified by regions that are created by the user with easy-to-use drawing tools, and combined using simple, logical formulas. Other sophisticated features include cell cycle analysis and peak analysis. Custom reports are quickly and easily generated from the acquired data using the integrated, macro-driven interface with Microsoft™ Word and Excel. FloMax data files are stored in FCS standard format and are therefore fully compatible with other commercial flow cytometry software.

The FloMax software is pre-installed on the Windows™ PC that is delivered as part of the CyFlow Space systems. Three additional licences for offline copies of the FloMax software are also included free of charge, which offers a cost-effective solution for working groups with the need for offline data analysis software.

Available options

Available options

CyFlow Space Autoloading Station
General facts
Automatic sample loading from 96-/384-well plates
High-speed mode < 15 minutes/96-well plate
User-defined cleaning and sample re-suspension
Start-up time < 2 minutes

Microplates
96- and 384-well plates with V-, U- and flat-bottom wells
Plates meeting the standards ANSI/SLAS (1-2004 through 4-2004) 

Sample volume
Flexible choice of sample volume from 10 to 150µL

Speed
Sample injection speed adjustable from 0.2µL/s to 10µL/s

Cycle time per microplate
a. High-speed mode < 15min
b. Balanced mode < 30min
c. Precision mode < 50 min
or customised by the operator

Dimensions
200 (W) x 280 (H) x 320 (D) mm

Applications

Applications

Absolute cell and particle counting
Cell and particle sorting

Microbiology
Yeast viability and fermentation process control
Water quality analysis
Absolute counting of bacteria and other microorganisms
Viability of bacteria and other microorganisms
Quality control in dairy industry and milk products
Marine biology and algae
Agrosciences and aquaculture
Quality control in cosmetics

Industrial applications
Bioreactor process optimisation
Quality assurance in food and beverage industry
Biomonitoring
Biofuels

Cell Biology
Cell cycle and DNA analysis
Viability and live/dead analysis
Cell proliferation
Apoptosis
Cancer research
Toxicology
Cytokines and bead-based assays
Cell signalling
Plant biology
Detection of ploidy level
Plant genome size and DNA analysis
Biomedical research
Animal models
Stem cells
Immunology
Haematological disorders
Platelets

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