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CyFlow Ploidy Analyser

  • Screening for Haplo-, Diplo-, Triplo- and Polyploids
  • Ploidy and Genome size analysis in less than two minutes
  • Detection of Anisoploids, Allopolyploids and Aneuploids
  • No time consuming microscopic evaluation
  • Any plant material can be analysed: leaves, seedlings, roots, flowers, peel of fruits, seeds

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Automatic measurement of ploidy Level and genome size in plants and animals

The CyFlow Ploidy Analyser is a compact flow cytometer for ploidy analysis, high-resolution DNA and genome size analysis for plants, animals and micro-organisms.

This modular system is equipped with a UV LED (365 nm) and/or a green laser (532 nm) which, when used in combination with Partec CyStain reagents, provides high-resolution DNA analysis with DAPI or propidium iodide.

The CyFlow Ploidy instrument measures the DNA content of many thousands of individual nuclei within seconds or minutes. The result is directly displayed as a DNA histogram on the internal 15.4" colour display. This method is time- and cost-saving as it provides precise results quickly with an efficient and validated workflow. For higher throughput, it is possible to equip the system with an Autoloading Station that accepts up to two 96-well plates or 120 test tubes as a single load.

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Specifications

Specifications

Light sources (up to two)
UV LED (365 nm)
Nd-YAG green laser at 532 nm (30 mW)

Optics
Standard set-up and filters for propidium iodide (long pass filter 590 nm) and/or DAPI/SSC (long pass filter 435 nm)

Flow system
Quartz flow cuvette for laminar sample transport and hydrodynamic focussing
True Volumetric Absolute Counting (TVAC) based on mechanical volume measurement
Completely closed fluidic systemSample port with biosafety cleaning system

Dimensions
385 (W) x 290 (H) x 280 (D) mm

Software

Software

CyView™: flow cytometry data acquisition and analysis software

  • Windows™-based FCM software CyView for real-time data acquisition, real-time data analysis and real-time data display
  • Easy set-up of experimental templates (configuration files)
  • Flow Cytometry Standard (FCS) data format for storage of original and evaluated data
Available options

Available options

CyFlow Robby 6 Autoloading Station
General facts
Protective housing with no light incidence
Flexible well/tube measurements

Microplates and tubes
48- or 96-well plates; V-, U- and flat-bottom are fitting (max. 2)
Plates meeting the standards ANSI/SLAS (1-2004 through 4-2004)
2mL-tubes (max. 120)

Sample volume
Flexible choice of sample volume up to 180 µL for plates and 2,000 µL for tubes

Speed
Continuously adjustable from 0.2 to 10 µL/s
Approx. 1h for a 96-well plate (speed 2 µL/s, sample volume 20 µL)

Dimensions
360mm (W) x 270mm (H) x 370 (D) mm

Applications

Applications

General
Plant and animal ploidy determination
Aneuploidy detection
Precise genome size estimation

For research and industrial applications
Biology and biotechnology
Seed and seedling industry
Plant taxonomy and population evolution / ecology

Sample preparation

Sample preparation

Celltrics (Cell strainers)
CyStain UV Ploidy (DAPI)
CyStain UV Precise P (DAPI)
CyStain UV Precise P automate (DAPI)
CyStain UV Precise T (DAPI)
CyStain UV Precise T automate (DAPI)
CyStain DNA 1 step (DAPI)
CyStain DNA 2 steps (DAPI)
CyStain PI Absolute P (PI)
CyStain PI Absolute T (PI)

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CyFlow Ploidy Analyser Brochure

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